FcγRIIB Mediates C-Reactive Protein Inhibition of Endothelial NO Synthase

Fc RIIB Mediates C-Reactive Protein Inhibition of Endothelial NO Synthase

C-reactive protein (CRP) is an acute-phase reactant that is positively correlated with cardiovascular disease risk and endothelial dysfunction. Whether CRP has direct actions on endothelium and the mechanisms underlying such actions are unknown. Here we show in cultured endothelium that CRP prevents endothelial NO synthase (eNOS) activation by diverse agonists, resulting in the promotion of mon...

C-reactive protein downregulates endothelial NO synthase and attenuates reendothelialization in vivo in mice.

C-reactive protein (CRP) is an acute-phase reactant that is positively associated with cardiovascular disease risk and endothelial dysfunction. In cell culture, CRP decreases the expression of endothelial NO synthase (eNOS), which regulates diverse endothelial cell (EC) functions including migration. To determine whether CRP alters EC gene expression and phenotype in vivo, we studied CF1 transg...

Inhibition of endothelial nitric oxide synthase by C-reactive protein: clinical relevance.

In 2002, 2 groups independently reported that C-reactive protein (CRP) inhibits endothelial nitric oxide synthase (eNOS) activity and bioactivity (1, 2 ). There was much reported debate as to whether these effects were artifacts attributable to CRP contamination with azide and endotoxin. In cells in which the endotoxin receptor Toll-like receptor 4 was knocked down by use of the small-interferi...

Inhibition of Experimental Autoimmune Encephalomyelitis in Human C-Reactive Protein Transgenic Mice Is FcγRIIB Dependent

We showed earlier that experimental autoimmune encephalomyelitis (EAE) in human C-reactive protein (CRP) transgenic mice (CRPtg) has delayed onset and reduced severity compared to wild-type mice. Since human CRP is known to engage Fc receptors and Fc receptors are known to play a role in EAE in the mouse, we sought to determine if FcγRI, FcγRIIb, or FcγRIII was needed to manifest human CRP-medi...

C-Reactive Protein Mediates Protection from